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Research Paper

MOLECULAR FINGERPRINTING OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) ISOLATES FROM A TERTIARY PHILIPPINE HOSPITAL

Keywords

staphylococcus aureus, phylogram, MRSA, randomly amplified polymorphic DNA (RAPD)

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) remains a principal risk to the community worldwide due to the ability of the MRSA clones to disseminate and diversify with increasing virulence, interchange of genetic lineages and antimicrobial resistance.  There are no investigative studies addressing the epidemiology of MRSA clones in the Philippines thru molecular diagnostic methods.  This study aimed to determine the molecular diversity of MRSA by randomly amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR).  MRSA isolates were collected from clinical specimens received from out-patients and hospitalized patients at Makati Medical Centre in Makati City, Philippines, between January 2013 to June 2013. All of the 108 MRSA strains in which the SCCmec and the pvl genes were detected by PCR were run to RAPD-PCR with a single primer. This primer generated polymorphisms in all the isolates and outcomes were summarized in a phylogram to reveal their relationships between the isolates.  The phylogram obtained from the analysis of RAPD analysis showed two major groups (Groups I–II) with several clusters in the second group. RAPD-PCR proved to be an easy yet helpful method for molecular typing, epidemiological investigation of outbreaks and assists health care administrators and providers in the formulation of effective control measures in the community and hospitals. 

Reference This Paper

MOLECULAR FINGERPRINTING OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) ISOLATES FROM A TERTIARY PHILIPPINE HOSPITALDemetrio L. Valle Jr., MD, MSc.International Journal of Microbiology and Parasitology, 1:1 (24 January 2015)

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